Hi, I just bought your book, and I’m very excited to get started. I’ve always wanted to be in “good shape,” lean and tone, but have never been able to get rid of jiggle. I’m curvy at 5’3″ and 132 pounds, and getting rid of fat in place of muscle is what I’m looking to do. I’ve always tried to remain active, even when I can’t go to the gym on a regular basis. For the past few weeks I’ve been working out twice a day, with heavy cardio and mild weight training in the morning, and interval circuit training in the evening while also doing a heavy HEAVY cut back on calories. I lost weight in my first week, but after that, it kind of just stuck near 132 and hovered. I’m much more concerned with my appearance than the number on the scale (though that doesn’t hurt either). I’m a little nervous that going from eating so few calories to what you recommend might cause a temporary fat gain. Also, I have a very busy schedule, but I make the time to make my own lunches and snacks to bring to work so that I can stay on track with whatever program I’m doing. Is there a meal plan provided in the book or just guidance tips to create your own? The book comes tomorrow, but I’m excited to get started and want to make sure I have all the tools I need to do so.
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It is established that bovine urine can result positive for boldenone and androstadienedione in consequence of faecal contamination. The simple transfer of steroids to urine is one minor aspect of faecal contamination. A high de novo production of steroids in faeces after deposition and in faeces-contaminated urine is almost certainly due to microbial activity, although the precursor compounds and transformations leading to the presence of these illegal steroids are unclear. We developed a simple in vitro method – incubation of faecal matter suspended in % saline – to induce steroid transformations in faeces, and analyzed the products by liquid chromatography/tandem mass spectrometry, without the need for prior extraction. Norethandrolone was the internal standard. The linearity (R 2 : –), sensitivity (LODs: to ng/mL; LOQs: to ng/mL), precision (intra-day CVs: –; inter-day CVs: –) and accuracy (percentage recovery: 89–120%) were calculated for the studied steroids. Androstenedione, androstadienedione, α - and β -boldenone, testosterone and epitestosterone transformations were investigated. Mutual interconversion of steroids was observed, although 17 β -hydroxy steroids had low stability compared with 17 α -hydroxy and 17-keto steroids. The results suggest that this simple in vitro system may be an effective way of studying hormone transformations in faeces and, after analogue studies, in faeces-contaminated urine. Copyright © 2007 John Wiley & Sons, Ltd.